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Xenopus animals are fed our custom frog diet, Nasco Frog Brittle, produced exclusively for Nasco by Purina Mills®. There is no wheat gluten or rice protein concentrate in this product. Meat meal source is porcine. It is not from ungulates.
Fish Meal, Porcine Meat and Bone Meal, Dehulled Soybean Meal, Ground Corn, Wheat Flour, Brewers Dried Yeast, Dried Egg Product, Glyceryl Monostearate, Corn Distillers Dried Grains with Solubles, Whey, Wheat Germ, Salt, Choline Chloride, Pyridoxine Hydrochloride, L-Ascorbyl-2 Polyphosphate (Stabilized Vitamin C), DL-Alpha Tocopheryl Acetate (Form of Vitamin E), Biotin, Cholecalciferol (Form of Vitamin D3), Vitamin A Acetate, Calcium Carbonate, Calcium Pantothenate, Menadione Sodium Bisulfite Complex (source of Vitamin K), Ethoxyquin (a Preservative), Thiamine Mononitrate, Folic Acid, Riboflavin Supplement, Nicotinic Acid, Vitamin B-12 Supplement, Manganous Oxide, Zinc Oxide, Ferrous Carbonate, Copper Sulfate, Zinc Sulfate, Calcium Iodate, Cobalt Carbonate, Sodium Selenite.
Caloric content of this animal diet is 2.8 K cal/gm. Meat meal source is porcine, not ungulate. POAO - Percentage of animal derived product in this food is at least 47.5%.
- Vitamin A, 13,585 I.U. per kg
- Vitamin D3, 2,090 I.U. per kg
- Vitamin E, 175 I.U. per kg
- Vitamin B12, 58 µg/kg
- Thiamin, 15 ppm
- Riboflavin, 11 ppm
- Pantothenic Acid, 34 ppm
- Niacin, 100 ppm
- Choline, 3,230 ppm
- Pyridoxine, 12 ppm
- Ascorbic Acid, 255 ppm
- Calcium, 3.7%
- Phosphorus, 2.2%
- Potassium, 1.2%
- Magnesium, 0.22%
- Sodium, 0.51%
- Chloride, 0.70%
- Iron, 510 ppm
- Copper, 16 ppm
- Manganese, 80 ppm
- Zinc, 115 ppm
- Iodine, 1.2 ppm
- Selenium, 0.09 ppm
Recommendations for Shelf Life:
All foods are packaged fresh for shipment. Kept dry in an air tight container at room temperature, the food is stable for 1 year. Shelf life is extended indefinitely if frozen.
Researchers Note: Your special protocol may suggest more frequent rotation.
Recommendation for Care of Xenopus Frogs, Adult
Optimal Temperature: 16° C - 18° C.
Optimal pH: Animals can tolerate a wide range of pH. Aim for somewhere in the 5 to 9 range, neutral preferred.
Light Cycle: 12 dark/12 light acceptable. For long-term care maintain under full spectrum fluorescent lights. Most commercial light manufacturers offer specialty tubes that would be suitable.
Density: Adult frogs should be allotted 1 gallon of water per frog.
Water: Use good quality water. If chlorine or chloramine are present, they should be removed from water prior to use. Not all water treatments or filtering systems can remove chloramine. Check with your municipal water department to see if chloramine has been added to your tap water. If you choose to use deionized water, you may wish to adjust osmotic balance by adding salts back into the water before use.
Do not expose animals to excessive fresh tap water. If you use a flow-through water exchange system, you will need to monitor incoming flow rates. Avoid sudden water temperature fluctuations. Animals can usually tolerate a 5° F increase or 10° F rapid decrease. Adult animals can slowly adjust to temperatures almost down to freezing and up to 90°+ F.
Change water as needed. Depending on the density, this may be once a week, twice a week, or on a daily basis. NOTE: A healthy frog will continuously shed skin. If you choose to incorporate a filtering system, you should plan to frequently change most filters because of this.
Food: Feed at a quiet time of the day when the animals are calm. Avoid disturbing them before or after. The frogs will either ignore the food in the first instance or regurgitate what they’ve consumed in the latter. We recommend cleaning tanks first thing in the morning and feeding late in the afternoon. The animals can digest their food during the night while they are calm. Animals will not eat if the tank is too dirty. We recommend feeding your colony Nasco’s Frog Brittle as a complete, balanced diet. Feed 1 gram/frog, twice a week.
Health: Xenopus are very robust amphibians. Of the few problems that might arise in the lab, most can be linked to stress or water quality. Many of the causative organisms are ubiquitous in standing water, but multiply out of control when water quality declines or the animal’s natural defenses are compromised by stress.
Female Xenopus for Oocyte Harvest: It is important to maintain cool temperatures (16°-18° C). Temperatures in the mid-twenties, even for a few hours, can leave the frogs unharmed but their oocyte quality jeopardized. Problems like this can occur in the summer during transit or if temperature controls fail in the lab. Oocytes will have weak membranes and/or spotty demarcations if this happens.
There can be seasonal variations. The animals go through a natural breeding phase around May through June. Hormone levels are higher in the females at this time and you may see fluctuations in the oocytes they produce.
“Old” oocytes can show up in virgin females that have never had an opportunity to lay eggs. To continue quality egg production, mature females should have ovulation induced every four months. Colonies where large numbers of frogs are held for long periods should schedule females through ovulation four to eight weeks prior to use. Seasonal variations in hormone levels and water temperatures will affect when a frog might rebuild stage 5 or 6 oocytes. There will be individual variations from frog to frog.
Ovulation can be induced by a single injection of 500 I.U. of HCG hormone into the dorsal lymph sac. Most frogs will shed eggs within 36 hours of injection.
Please contact us for further information or to place your order.
Debra Tipton, Biology Operations Manager
Phone: 1.920.568.5565 (for information)
Phone: 1.800.558.9595 (to place an order)
Email: [email protected]