- An instrument fitted with objectives and an eyepiece lens and used for viewing small objects at high magnifications.
- Light is passed through the specimen and it transmits the image through the objectives and into the eyepiece.
- The image will appear upside down and reversed from left to right.
How does it work?
- Place a prepared slide onto the stage using the stage clips to secure it. If the microscope has a mechanical stage, allow it to come into contact with the slide.
- Always start with the nosepiece in the lowest powered objective.
- Turn the focus knobs so that the stage and objective are as close together as they will go.
- Turn the illuminator to the "on" position. Light should come from the sub-stage illuminator.
- Look through the eyepiece and slowly turn the coarse focus knob so that the stage and objective are moving away from each other.
- Continue until the image appears.
- Once the image is in focus, use the fine focus (if available) to focus the image clearly.
- After the image is in focus, switch to a higher power objective.
- Adjust the diaphragm to either Disc or Iris to manipulate the amount of light that passes through the image. This will increase the clarity of your image.
- 100X Oil Immersion Objective
- Follow above steps until ready to switch to the 100X objective (Oil Immersion Objective)
- Rotate the nosepiece so that the 100X objective is pointing toward the stage
- Focus the image
- Switch the objective out of the light path and place one drop of immersion oil unto the slide.
- Swing the 100X objective back over the slide. The oil should form a little column from the objective to the slide with no space in between
- When finished using the 100X objective, wipe off the oil from the objective. Do not let the oil dry. This will decrease visibility and eventually ruin your objective
- Once the oil is placed on the slide, do not switch objectives.
- Microscope Heads
- Monocular One ocular tube that can be a straight tube at a 90° angle or 45° inclination. Most are rotatable 360°.
- Dual 45° head Two ocular tubes that are both set at 45° inclinations. Allows two people to view the microscope simultaneously.
- 90° dual head Two ocular tubes, one at 45° and the other at 90°. Generally used for video microscopy.
- Binocular head Two eyepieces used for prolonged viewing.
- Microscope Stages
- Plain A standard stage with two stage clips. Use fingers to move the slide around.
- Mechanical Stage Able to move slide by turning two knobs in the y-axis and x-axis.
- Built-in Mechanical Stage Entire stage moves when the controls are turned.
- Microscope Illuminators
- Tungsten "Incandescent light". Most common and cheapest. Gives off a yellowish color and gives off extra heat.
- Fluorescent Heat given off is very minimal and has a cool bright, white light.
- Halogen Found in most advanced microscopes. It's the brightest light but gives off excess heat.
- LED Newest lighting option. Rechargeable batteries power the LED light. Produces a very bright, white light and less heat than a fluorescent bulb.