Specificity of Albumin Binding
Product Number: SB49182
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The binding of an enzyme to its substrate is only one example of the many specific molecular interactions that occur in biological systems. An analogous binding process occurs with serum albumin, which binds certain small molecular weight compounds and serves as a carrier molecule for these compounds in blood. In this exercise, students use an electrophoretic assay to examine the binding of various dyes to albumin. The results of this graphic analysis show that the binding of dyes to albumin is saturable, specific, compatible, and dependent on the native structure of the protein. The exercise is designed such that each of the eight groups of students performs a different experiment. Each group then describes their results and conclusion to the entire class. This exercise is a valuable experience in analyzing data and provides a fine introduction to enzyme kinetics. Contents include complete instructions, bovine serum albumin, dilution buffer, bromophenol blue, orange G, ponceau S, sodium dodecyl sulfate, rabbit hemoglobin, 5 g agarose, and enough TG buffer to make 3.5 liters. You will need electrophoresis equipment. For 16 students working in pairs over two one-hour long lab periods.