Edvotek® AP® Curriculum II - Lab 9: Biotechnology - Restriction Enzyme Analysis of DNA
Product Number: SB49289
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Offer students an opportunity to get the most out of their AP® biology class with a lab that reflect the standards of the revised AP® Biology program. This introductory college level lab, designed for the more advanced biology student, provides plenty of opportunities to develop scientific inquiry and reasoning skills while practicing proper lab skills. High school. Contains 8 gels (2-3 students each), 16-24 students total.
This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield six bands (five distinct bands, two very close in size) corresponding to the DNA fragments.