Innovating Science® AP® Biology Lab - Investigation #8: Bacterial Transformation

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Product Number: SB49649

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Grades 9-12. Allow students to get the most out of their AP® Biology class with college-level labs designed to challenge the more advanced student. Students will explore key concepts while preparing for higher education. Enough materials provided for eight lab groups.

Genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance. Examine the number of resistant bacteria to determine the efficiency of the transformation procedure. Redeem coupon to receive required perishable materials. Includes teacher’s manual and student study guide copy masters. Meets AP® Science Practices 1, 3, 5, 6, and 7, and Big Idea 3.

Kit includes:

  • 30 ml sterile calcium chloride 0.05M
  • 8 pipet bulbs
  • 16 microcentrifuge tubes, 1.5 ml
  • 40 Petri dishes
  • 9 ml tube Luria broth
  • 4 Luria agar, 200 ml
  • 16 sterile volumetric pipettes
  • 8 micopipettes with plungers, 10µl

Materials sent upon redemption of included coupon:

  • 2 bottles 0.02 g ampicillin
  • 1,000 ng pUC 19 plasmids
  • 9 ml tryptic soy broth tube
  • 6 ml tryptic soy agar slants
  • Sterile, disposable pipet
  • Freeze-dried E. coli culture

Materials needed but not supplied:

  • Large beaker
  • Hot plate or water bath
  • Microwave (not necessary if melting agar in a water bath)
  • Markers
  • Microfuge tube racks
  • Container of ice


  • Incubator (37° C)

Item Weight: 5.50
Age: 14 yrs. - 18 yrs.
Prop 65:
Assembly Required: false
Grade: 9 - 12
School Type: High School
Subject Areas: Biology


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