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Edvotek® AP Lab 6B: Transformation of E. Coli
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Offer students an opportunity to get the most out of their AP biology class with this lab designed for the more advanced biology student. Help your students prepare for higher education as they learn the core concepts of this innovative and exciting introductory college level lab. High school. Contains enough materials for up to 10 groups.
In this experiment, transformed cells take up a plasmid containing the GFP gene. The GFP was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to long wave UV light. Kit includes cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ Agar, Luria broth, petri plates, pipets, inoculating loops, microtest tubes, and instructions. Plating takes five minutes, cells require overnight incubation, and transformation efficiency requires 15 minutes.
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