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Edvotek® AP Lab 6A: Analysis of Eco RI Cleavage Patterns of Lambda DNA
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Offer students an opportunity to get the most out of their AP biology class with this lab designed for the more advanced biology student. Help your students prepare for higher education as they learn the core concepts of this innovative and exciting introductory college level lab. High school. Contains enough materials for up to 10 groups.
This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and six Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield six bands (five distinct bands, two are very close in size) corresponding to the DNA fragments. Kit includes Ready-to-Load™ DNA samples, UltraSpec Agarose™ powder, practice gel loading solution, electrophoresis buffer, InstaStain® Methylene Blue and Methylene Blue Plus™ stain, calibrated pipet, 100 ml graduated cylinder, micro-tipped transfer pipets, and instructions. For six gels. Lab completed in approximately 60 minutes.
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